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AB2281

Sigma-Aldrich

Anti-Na+K+Cl- Cotransporter 2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter, NKCC2A variant A, Na-K-2Cl cotransporter, sodium potassium chloride cotransporter 2, solute carrier family 12 (sodium/potassium/chloride transporters), m

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

technique(s)

western blot: suitable

NCBI accession no.

NM_000338.2

UniProt accession no.

Q13621

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC12A1(6557)

General description

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle′s loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. SLC12A1 is kidney specific and is essential in the regulation of ionic balance and cell volume. It is a component of an electrically silent transporter system, mediating sodium and chloride reabsorption. Defects in SLC12A1 are the cause of Bartter syndrome type 1, an autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria.

Specificity

This antibody recognizes the cytoplasmic domain of NA+K+CL- Cotransporter 2.

Immunogen

Epitope: Cytoplasmic domain
KLH conjugate followed by NA+K+CL- Cotransporter 2 corresponding to the cytoplasmic domain.

Application

Anti-Na+K+Cl-Cotransporter 2 Antibody detects level of Na+K+Cl-Cotransporter 2 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Quality

Western Blot Analysis:
1:2,000 dilution of this lot detected NA+K+CL- Cotransporter 2 on 10 µg of rat liver lysate.

Target description

~150 kDa

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Rat liver lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Whey protein hydrolysate increases translocation of GLUT-4 to the plasma membrane independent of insulin in wistar rats.
Morato, PN; Lollo, PC; Moura, CS; Batista, TM; Camargo, RL; Carneiro, EM; Amaya-Farfan, J
Testing null
Meng-Hsuan Lin et al.
JCI insight, 6(20) (2021-09-10)
The prevailing view is that the ClC-Ka chloride channel (mouse Clc-k1) functions in the thin ascending limb to control urine concentration, whereas the ClC-Kb channel (mouse Clc-k2) functions in the thick ascending limb (TAL) to control salt reabsorption. Mutations of
Chih-Chien Sung et al.
Frontiers in medicine, 8, 679171-679171 (2021-06-29)
Background: The utility of urinary extracellular vesicles (uEVs) to faithfully represent the changes of renal tubular protein expression remains unclear. We aimed to evaluate renal tubular sodium (Na+) or potassium (K+) associated transporters expression from uEVs and kidney tissues in

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