AB16969

Anti-Caspase 9 Antibody, proform & lg. subunit

Chemicon^®, from rabbit

• Synonym(s): ICE-LAP6, Mch-6, Apaf-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human
• should not react with: rat
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_001229.2; NM_032996.1
• UniProt accession no.: P55211
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP9(842)

Description

Specificity

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (Nijhawan et al. 1997). Caspase-9 is also activated by granzyme B and CPP32 (Duan et al. 1996; Srinivasula et al. 1996). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Nijhawan et al. 1997). Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis inducers including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD (Pan et al. 1998). Caspase-9 is expressed in a variety of human tissues (Duan et al. 1996; Srinivasula et al. 1996).

Immunogen

Epitope: proform & lg. subunit

Synthetic peptide corresponding to amino acids 41-56 of human caspase-9 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Recognizes proform (46 kDa); sequence analysis predicts cross-reactivity with mouse caspase-9 (>90%).

Application

Detect Caspase 9 using this Anti-Caspase 9 Antibody, proform & lg. subunit validated for use in WB.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

Western blot: 1:1,000 to 1:2,000

Whole cell lysate from HeLa cells can be used as a positive control and a 46 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-443

Physical form

Format: Purified

Immunoaffinity chromatography purified immunoglobulin. Liquid in PBS, containing 0.02% sodium azide.

Storage and Stability

The undiluted antibody solution is stable for approximately 6 months when stored 2-8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable