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219404

Sigma-Aldrich

Cathepsin H, Human Liver

Cathepsin H, Human Liver, CAS 60748-73-4, is native cathepsin H from human liver that functions both as an aminopeptidase and as an endopeptidase.

Synonym(s):

Cathepsin H, Human Liver, CTSH, cat H, procathepsin h

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About This Item

CAS Number:
Enzyme Commission number:
UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

form

liquid

specific activity

≥200 mU/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

technique(s)

activity assay: suitable

color

colorless

pH

4.5

solubility

soluble
water: soluble

suitability

suitable for Western blot

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−70°C

Gene Information

human ... CTSH(1512)

General description

Research area: Cell signaling

Native Cathepsin H, from the human liver, is the only cathepsin with mono-aminopeptidase activity.

Application

Cathepsin H has been used to determine the potential of aziridinyl peptides to act as inhibitors of this protein and potentially explore their therapeutic applications.

Biochem/physiol Actions

Cathepsin H, derived from human liver, serves dual functions as both an aminopeptidase and an endopeptidase. It plays a significant role in the catabolism of proteins within lysosomes. Cathepsin H is classified as an endoaminopeptidase because it can hydrolyze proteins, N-terminal substituted proteins, amino acid derivatives, and peptides with different chain lengths and N-terminal free amino acid derivatives. This protein is also observed to exhibit amidase and esterase activity.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol H-Arg-βNA per min at 40°C, pH 6.8.

Physical form

In 100 mM NaCl, 20 mM sodium acetate buffer, 1 mM EDTA, pH 4.5.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Note: 1 mU = 1 milliunit.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cathepsin H: an endoaminopeptidase from rat liver lysosomes
Acta Biologica Et Medica Germanica, 36(2), 185-199 (1977)
New peptidic cysteine protease inhibitors derived from the electrophilic alpha-amino acid aziridine-2,3-dicarboxylic acid
Schirmeister T
Journal of Medicinal Chemistry, 42, 560-572 (1999)

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