1.03854
GranuCult® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar
acc. FDA-BAM, ISO 21872
Synonym(s):
TCBS Agar, Thiosulfate Citrate Bile Salts Sucrose Agar, Vibrio Selective Agar
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Agency
ISO 21872
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
8.6±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
15-25°C
suitability
selective and differential for Vibrio spp.
General description
GranuCult® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar is a complex medium designed for the isolation and differentiation of Vibrio spp. and other enteropathogenic microorganisms from products intended for human consumption, animal feed, environmental samples in the production area, food production, food handling, and other materials.
The synergistic effect of high concentration of thiosulfate, citrate and strong alkalinity inhibits the growth of Enterobacteriaceae spp. The medium is highly selective as the bile salts present in the medium suppresses the growth of Enterococci spp.
The differentiation is achieved based on sucrose fermentation, pH indicators and H2S production from thiosulfate. Vibrio spp. do not produce H2S but depending on the species, may ferment sucrose. Peptone and yeast extract in the media allow good growth and agar is the solidifying agent.
The synergistic effect of high concentration of thiosulfate, citrate and strong alkalinity inhibits the growth of Enterobacteriaceae spp. The medium is highly selective as the bile salts present in the medium suppresses the growth of Enterococci spp.
The differentiation is achieved based on sucrose fermentation, pH indicators and H2S production from thiosulfate. Vibrio spp. do not produce H2S but depending on the species, may ferment sucrose. Peptone and yeast extract in the media allow good growth and agar is the solidifying agent.
Application
GranuCult® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar is used to isolate and differentiate Vibrio spp. and other enteropathogenic microorganisms from products intended for human consumption, animal feed, environmental samples in the production area, food production, food handling, and other materials.
Analysis Note
- The dehydrated medium is a granulate with green color.
- The prepared medium is clear to slightly opalescent and blue-green.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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