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D17106

Sigma-Aldrich

2,7-Diaminofluorene

>97%

Synonym(s):

2,7-Fluorenediamine

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About This Item

Empirical Formula (Hill Notation):
C13H12N2
CAS Number:
525-64-4
Molecular Weight:
196.25
Beilstein:
2099859
EC Number:
208-377-5
MDL number:
MFCD00001128
UNSPSC Code:
12352100
PubChem Substance ID:
24893519
NACRES:
NA.22

Quality Level

100

Assay

>97%

form

powder

mp

160-162 °C (lit.)

SMILES string

Nc1ccc-2c(Cc3cc(N)ccc-23)c1

InChI

1S/C13H12N2/c14-10-1-3-12-8(6-10)5-9-7-11(15)2-4-13(9)12/h1-4,6-7H,5,14-15H2

InChI key

SNCJAJRILVFXAE-UHFFFAOYSA-N

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Application

Analytical reagent for metals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000279676
0000201060
07204EH
09311DH
08009BH

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S J Gebran et al.
Journal of immunological methods, 151(1-2), 255-260 (1992-07-06)
A sensitive and rapid colorimetric method for the in vitro determination of phagocytic activity and antibody-dependent cell-mediated cytotoxicity (ADCC) is described. The assay uses red blood cells (RBC) as target cells and relies on the specific oxidation of 2,7-diaminofluorene (DAF)
B A Callus et al.
Hemoglobin, 19(1-2), 7-19 (1995-01-01)
We have used the diaminofluorene stain to detect hemoglobin production in J2E cells following erythropoietin-induced differentiation. The pseudo-peroxidase activity of hemoglobin produces a colored product, fluorene blue, which can be measured spectrophotometrically. We found that the absorbance varied with time
Colin P McGuckin et al.
European journal of haematology, 70(2), 106-114 (2003-02-13)
We have combined in vitro clonogenic culture and a highly sensitive stain for haemoglobin to compare the influence of EPO, IL-3, SCF, TGFbeta1, MIP-1alpha and IFNgamma, to directly stimulate cells in the progenitor compartment to develop towards the erythroid lineage.
Daniel Trnka et al.
Nature communications, 11(1), 3445-3445 (2020-07-12)
Despite their very close structural similarity, CxxC/S-type (class I) glutaredoxins (Grxs) act as oxidoreductases, while CGFS-type (class II) Grxs act as FeS cluster transferases. Here we show that the key determinant of Grx function is a distinct loop structure adjacent
R E Worthington et al.
Experimental hematology, 15(1), 85-92 (1987-01-01)
We have developed a new colorimetric method to detect early erythroid clonogenic progenitor cells by virtue of their hemoglobin-containing progeny in vitro. The method relies upon the specific oxidation of 2,7-diaminofluorene (DAF) by the pseudoperoxidase activity of hemoglobin (Hb). Generation
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