HL60 Ast.4
88120802, human blood, Promyeloid, blast/promyelocyte
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About This Item
UNSPSC Code:
41106514
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product name
HL60 Ast.4, 88120802
biological source
human blood
growth mode
Suspension
karyotype
2n = 48, hyperdiploid
morphology
Promyeloid, blast/promyelocyte
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian promyelocytic leukaemia
Cell Line Description
Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.
Application
Differentiation studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic.
Other Notes
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Certificates of Analysis (COA)
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