C22 (Clara)

7021401, mouse lung, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: C22 (Clara), 07021401, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: mouse lung
• packaging: tube of 5 μg 07021401-DNA-5UG; pkg of vial of cells 07021401-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse lung epithelial progenitor cell

Cell Line Description

A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Culture Medium

DMEM + 2 mM Glutamine + 4% Fetal Bovine Serum (FBS) + 8 supplements:

0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3

For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.

Subculture Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.