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Product Name
C22 (Clara), 07021401
biological source
mouse lung
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse lung epithelial progenitor cell
Cell Line Description
A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.
Culture Medium
DMEM + 2 mM Glutamine + 4% Fetal Bovine Serum (FBS) + 8 supplements:
0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3
For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.
0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3
For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.
Subculture Routine
Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;10% CO2; 33°C. Doubling time 48h
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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