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A9771

Sigma-Aldrich

Albumin–fluorescein isothiocyanate conjugate

protein bovine

Synonym(s):

FITC-albumin

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About This Item

MDL number:
UNSPSC Code:
12352204
eCl@ss:
42010201
NACRES:
NA.32

biological source

protein from bovine

form

powder

extent of labeling

≥7 mol FITCmol albumin

technique(s)

ELISA: suitable

solubility

10 mM Tris pH 7.0: 10 mg/mL

application(s)

detection

storage temp.

2-8°C

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General description

Albumin makes up 60% of the plasma protein, which is produced by the liver. It regulates colloid osmotic pressure. Albumin is implicated in the transportation, distribution and metabolism of fatty acids, thyroid hormones and drugs. It reduces weight gain, blocks pulmonary edema, decreases ascites and lowers tissue edema.

Application

Albumin−fluorescein isothiocyanate conjugate has been used:
  • as a control to compare the intracellular delivery efficiency of proteins with or without carrier
  • for the fluorescent visualization of proteins and cells
  • as a blocking agent in ELISA
  • as a chemical for brain perfusion and fixation
  • for in vivo capillary–epithelial permeability experiment
  • for confocal microscopy of right ventricular (RV) vasculature in rats

Preparation Note

Prepared from crystallized and lyophilized albumin (A 4378).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Antonio Brante et al.
The Journal of experimental biology, 212(Pt 14), 2287-2295 (2009-06-30)
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Tian L, et al
Journal of Molecular Medicine, 95(4), 381-393 (2017)
Molecular mechanisms of proteinuria
Molecular and Genetic Basis of Renal Disease (2008)
Whole brain vascular imaging in a mouse model of Alzheimer?s disease with two-photon microscopy
Delafontaine-Martel P, et al.
Journal of Biomedical Optics, 23(7), 076501-076501 (2018)
What is the best way to fluid-resuscitate a patient with sepsis?
Evidence-Based Practice of Critical Care, 198-205 (2010)

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