A3010

Acylase I from porcine kidney

Grade I, lyophilized powder, ≥1500 units/mg protein

• Synonym(s): Aminoacylase, N-Acylamino acid amidohydrolase
• CAS Number: 9012-37-7
• Enzyme Commission number: 3.5.1.14 (BRENDA, IUBMB)
• EC Number: 232-732-3
• MDL number: MFCD00081285
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Grade I
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥1500 units/mg protein
• composition: Protein, ≥60%
• UniProt accession no.: P37111
• storage temp.: −20°C
• Gene Information: pig ... ACY1(396930)

Description

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .

Unit Definition

One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves