Please consider that the condition of the stock enzyme is different from the reaction in which it is used. You will be using a small amount of enzyme in a buffered reaction that is adjusted to the optimal pH by the reaction buffer itself.
A0810
Endoglycosidase H from Streptomyces plicatus
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
β-N-Acetylglucosaminidase H
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
recombinant
expressed in E. coli
Quality Level
conjugate
(N-linked)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
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General description
Endoglycosidase H or endo-β-N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.[1]
Biochem/physiol Actions
Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.[2]
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.
Physical form
Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTA
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Could you provide guidance on adjusting the pH of a solution that has a pH of 7.5, even though the workable pH range mentioned in the data sheet is 5.0–6.0? Specifically, which acid would be best to use for adjusting the pH before using the solution?
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