Skip to Content
Merck
All Photos(1)

Key Documents

11418467001

Roche

Chymotrypsin Sequencing Grade

from bovine pancreas

Synonym(s):

chymotrypsin, protease

Sign Into View Organizational & Contract Pricing


About This Item

Enzyme Commission number:
UNSPSC Code:
12352204

biological source

bovine pancreas

Quality Level

Assay

≥90%

form

lyophilized (salt-free)

specific activity

≥70 units/mg protein (at 25 °C, with ATEE as a substrate)

mol wt

Mr 25 kDa

packaging

pkg of 4 × 25 μg

manufacturer/tradename

Roche

technique(s)

protein sequencing: suitable

optimum pH

7.0-9.0

shipped in

wet ice

storage temp.

2-8°C

General description

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Specificity

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Application

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Quality

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Preparation Note

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstitution

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl2, pH 7.8).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Virus-encoded proteinases and proteolytic processing in the Nidovirales.
J Ziebuhr et al.
The Journal of general virology, 81(Pt 4), 853-879 (2000-03-22)
A method for selective 19 F-labeling absent of probe sequestration (SLAPS).
Dixon, et al.
Protein Science, 31, e4454-e4454 (2023)
Wei-Qiang Chen et al.
Amino acids, 44(5), 1381-1389 (2013-03-21)
The protocol consists of running a native gel with in-gel digestion by proteases, subsequent mass spectrometrical determination of protein sequence and modifications, followed by electro-elution and conformational analysis using melting point and circular dichroism. Finally, the eluted protein is tested
Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product.
Zacchi, et al.
Communications biology, 4, 390-390 (2021)
Uyen T T Nguyen et al.
Current protocols in protein science, Chapter 14, Unit14-Unit14 (2010-11-26)
Post-translational modifications (PTMs) expand the number of protein isoforms in eukaryotic proteome by orders of magnitude. Protein modification with isoprenoid lipids is a common PTM affecting hundreds of proteins controlling the transport of information and materials into, through, and out

Articles

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service