10295892001

Colcemid

solution, suitable for blocking, sterile; 0.2 μm filtered

• Synonym(s): Demecolcine, N-Deacetyl-N-methylcolchicine
• Empirical Formula (Hill Notation): C₂₁H₂₅NO₅
• CAS Number: 477-30-5
• Molecular Weight: 371.43
• Beilstein: 2822892
• MDL number: MFCD00075459
• UNSPSC Code: 12352207
• PubChem Substance ID: 329749009

Properties

• description: N-methyl-N-deacetyl-colchicine
• Quality Level: 100
• sterility: sterile; 0.2 μm filtered
• form: solution
• packaging: pkg of 20 mL (10 μg/ml)
• manufacturer/tradename: Roche
• technique(s): blocking: suitable
• impurities: microbial, tested
• solubility: water: miscible
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
• InChI key: NNJPGOLRFBJNIW-HNNXBMFYSA-N

Description

General description

Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.

Application

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis.

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G₁ and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Physical form

Solution (10 μg/ml), filtered through 0.2 μm pore size membrane.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash