MAB5492
Anti-Huntingtin Antibody, a.a. 1-82
ascites fluid, clone 2B4, Chemicon®
Synonym(s):
Anti-Huntingtin, Anti-Huntingtin Antibody, Detection Antibody
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2B4, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HTT(3064) , SLC6A4(6532)
Specificity
Reacts huntingtin protein, amino acids 1-82. The antibody recognizes wild type and mutant huntingtin.
Immunogen
Epitope: a.a. 1-82
Recombinant human huntingtin, amino acids 1-82.
Application
Anti-Huntingtin Antibody, a.a. 1-82 is an antibody against Huntingtin for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Western blot: 1:500-1:5,000
Immunocytochemistry (1): 1:500-1:5,000
Immunohistochemistry (1,2): 1:500-1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by end user.
Immunocytochemistry (1): 1:500-1:5,000
Immunohistochemistry (1,2): 1:500-1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by end user.
Target description
348 kDa
Physical form
Ascites fluid containing no preservatives.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Normal human cerebral cortex lysate, Mouse brain cortex samples from HD or wild type mice
Normal human cerebral cortex lysate, Mouse brain cortex samples from HD or wild type mice
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Phosphorylation of mutant huntingtin at serine 116 modulates neuronal toxicity.
Watkin, EE; Arbez, N; Waldron-Roby, E; O'Meally, R; Ratovitski, T; Cole, RN; Ross, CA
Testing null
Sean M DeGuire et al.
The Journal of biological chemistry, 293(48), 18540-18558 (2018-09-07)
Huntington's disease is a fatal neurodegenerative disorder resulting from a CAG repeat expansion in the first exon of the gene encoding the Huntingtin protein (Htt). Phosphorylation of this protein region (Httex1) has been shown to play important roles in regulating
Sophie Vieweg et al.
The Journal of biological chemistry, 291(23), 12074-12086 (2016-03-24)
The first exon of the Huntingtin protein (Httex1) is one of the most actively studied Htt fragments because its overexpression in R6/2 transgenic mice has been shown to recapitulate several key features of Huntington disease. However, the majority of biophysical
Gennady Ermak et al.
The Journal of biological chemistry, 284(18), 11845-11853 (2009-03-10)
Our work suggests an important new link between the RCAN1 gene and Huntington disease. Huntington disease is caused by expansion of glutamine repeats in the huntingtin protein. How the huntingtin protein with expanded polyglutamines (mutant huntingtin) causes the disease is
S-nitrosylation of dynamin-related protein 1 mediates mutant huntingtin-induced mitochondrial fragmentation and neuronal injury in Huntington's disease.
Haun, F; Nakamura, T; Shiu, AD; Cho, DH; Tsunemi, T; Holland, EA; La Spada, AR; Lipton, SA
Antioxidants & Redox Signaling null
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