Skip to Content
Merck
All Photos(1)

Key Documents

FCMAB176F

Sigma-Aldrich

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25

clone DK25, Milli-Mark®, from mouse

Synonym(s):

Anti-CD8 FITC, Clone DK25 Anti-CD8

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

DK25, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD8A(925)

Related Categories

General description

CD8 is a 68 kDa disulphide-linked, transmembrane glycoprotein. The CD8 molecule serves as a receptor for MHC class I molecules and can mediate a function as a coreceptor in TCR-ligand binding and T-cell activation. Its cytoplasmic part is associated with the p56Ick tyrosine kinase (Nakuchi, 1997). CD8 is expressed by the great majority of cortical thymocytes and approximately 30% of medullary thymocytes, and by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells. In addition a proportion of gamma delta T cells and NK cells express CD8 (Nakuchi, 1997). Thus, CD8 is expressed by about 25-35% of peripheral T cells, and about 30% of NK cells express low levels of CD8 (Leong, 1999). The specificity of Anti-CD8, DK25, is equivalent to that of the CD8-clustered antibodies OKT8 and Leu-2a (Stein, 1984).

Specificity

Antibody recognizes Human CD8 antigen.

Application

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25 detects level of CD8 & has been published & validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by flow cytomatry using human lymphocytes.

Target description

68 kDa (Nakuchi, 1997)

Physical form

Protein A purified
Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Human Lympnocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kim J Hasenkrug et al.
mBio, 12(4), e0150321-e0150321 (2021-07-28)
Severe coronavirus disease 2019 (COVID-19) has been associated with T cell lymphopenia, but no causal effect of T cell deficiency on disease severity has been established. To investigate the specific role of T cells in recovery from severe acute respiratory
Nicole Marzan-Rivera et al.
iScience, 25(8), 104764-104764 (2022-08-20)
The link between CD4+ T and B cells during immune responses to DENV and ZIKV and their roles in cross-protection during heterologous infection is an active area of research. Here we used CD4+ lymphocyte depletions to dissect the impact of
Shu-Qi Zhang et al.
Science translational medicine, 8(341), 341ra77-341ra77 (2016-06-03)
T cells recognize and kill a myriad of pathogen-infected or cancer cells using a diverse set of T cell receptors (TCRs). The affinity of TCR to cognate antigen is of high interest in adoptive T cell transfer immunotherapy and antigen-specific

Articles

Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.

Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.

Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.

Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.

See All

Protocols

Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service