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P3775

Sigma-Aldrich

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Anti-Protein A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

packaging

vial of 2 mL lyophilized antiserum

technique(s)

indirect ELISA: 1:100,000

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

Immunogen

Protein A from Staphylococcus aureus

Application

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.
Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Biochem/physiol Actions

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Physical form

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Reconstitution

Reconstitute with 2 ml deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ritu Gupta et al.
PloS one, 10(7), e0132350-e0132350 (2015-07-07)
Saccharomyces cerevisiae Sub1 is involved in several cellular processes such as, transcription initiation, elongation, mRNA processing and DNA repair. It has also been reported to provide cellular resistance during conditions of oxidative DNA damage and osmotic stress. Here, we report
IMMUNOASSAY CONFIGURATIONS
Immunoassay (1996)
Shintaro Kira et al.
Autophagy, 10(9), 1565-1578 (2014-07-22)
Autophagy is an intracellular degradation process that delivers cytosolic material to lysosomes and vacuoles. To investigate the mechanisms that regulate autophagy, we performed a genome-wide screen using a yeast deletion-mutant collection, and found that Npr2 and Npr3 mutants were defective
Radioimmunoassay
Immunoassay, 269-285 (1996)
Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit
Torres MP, et al.
The Journal of Biological Chemistry, 286(23), 20208-20216 (2011)

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