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LYSISO1

Sigma-Aldrich

Lysosome Isolation Kit

sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells

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About This Item

EC Number:
233-140-8
UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

usage

sufficient for 20 mL (packed cells)
 sufficient for 25 g (tissue)

technique(s)

centrifugation: suitable
fractionation: suitable

storage temp.

2-8°C

General description

Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.

Application

Lysosome Isolation Kit has been used:
  • in the isolation of cytosol and lysosome
  • to obtain enriched light and heavy lysosomal fractions from cultured cells
  • in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
  • in lysosome purification

The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.

Features and Benefits

  • Generates functional organelles for metabolism and disease research
  • Includes protease inhibitor to manage degradation
  • Measures integrity with included Neutral Red dye
  • Choose from multiple options to obtain enriched fractions
  • Enriched fractions for intact and functional lysosomes from tissues and cells
  • Suitable for functional studies including protein degradation

Kit Components Also Available Separately

Product No.
Description
SDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDS

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fang-Shin Nian et al.
Molecular neurobiology, 56(9), 6095-6105 (2019-02-06)
Mutations in RAB18, a member of small G protein, cause Warburg micro syndrome (WARBM), whose clinical features include vision impairment, postnatal microcephaly, and lower limb spasticity. Previously, our Rab18-/- mice exhibited hind limb weakness and spasticity as well as signs
Xiaowei Ma et al.
ACS nano, 5(11), 8629-8639 (2011-10-07)
Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. Autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. Previous studies have shown that nanoparticles from various sources
Facilitated mitochondrial import of anti-viral and anti-cancer nucleoside drugs by human equilibrative nucleoside transporter-3 (hENT3)
Govindarajan R, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology (2009)
C Oberle et al.
Cell death and differentiation, 17(7), 1167-1178 (2010-01-23)
Apoptotic stimuli have been shown to trigger lysosomal membrane permeability (LMP), leading to the release of cathepsins, which activate death signaling pathways in the cytosol. However, it is unknown whether this process is an initiating or amplifying event in apoptosis.
Activation of Nlrp3 inflammasomes enhances macrophage lipid-deposition and migration: implication of a novel role of inflammasome in atherogenesis
Li X, et al.
PLoS ONE, 9(1), e87552-e87552 (2014)
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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