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H4385

Sigma-Aldrich

Hanks′ Balanced Salt solution

10 ×, Modified, without calcium, magnesium or sodium bicarbonate

Synonym(s):

HBSS

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About This Item

UNSPSC Code:
12352107
NACRES:
NA.47
Pricing and availability is not currently available.

Quality Level

200

concentration

10 ×

pH

5.8-6.0

application(s)

hematology
histology

storage temp.

room temp

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General description

Hanks′ Balanced Salt solution (HBSS) is a cell and tissue culture solution. It is widely recommended for cell maintenance and conservation of tissues for a longer period. In addition, HBSS is used as a wet storage media for avulsed teeth, as it has the ideal osmolality and physiological pH for preserving the vitality of cells.[1]

Application

Hanks′ Balanced Salt solution (HBSS) has been used for the elimination of the epithelial layer from the leukocytes and lymphocytes from the colon cells[2]. It has also been used for the isolation of quail spermatozoa[3]

Packaging

Contains: NaCl, 80 g/L; KCl, 4 g/L; glucose, 10 g/L; KH2PO4 600 mg/L; Na2HPO4 475 mg/L and phenol red, 170 mg/L

Other Notes

pH 6.9 when diluted according to instructions. Osmolality 240-250 mOsm/Kg.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ1820
SLCK3632
SLCG9310
SLCB6544
SLBV9986

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Shusei Mizushima et al.
Methods in molecular biology (Clifton, N.J.), 1650, 243-257 (2017-08-16)
A characteristic biological property of avian gamete (e.g., extremely large egg and polyspermic fertilization) does not allow the direct observation of sperm-egg interactions in vitro, but recent research advances make it possible to manipulate the gamete in vitro. Here, we
Wilson Roberto Poi et al.
Brazilian dental journal, 24(5), 437-445 (2014-01-30)
Dental avulsion is the most severe type of traumatic tooth injuries because it causes damage to several structures and results in the complete displacement of the tooth from its socket in the alveolar bone. The ideal situation is to replant
Györgyi Műzes et al.
Comparative immunology, microbiology and infectious diseases, 52, 48-57 (2017-07-05)
The influence of cell-free DNA (fDNA) administration on the TLR9-autophagy regulatory crosstalk within inflammatory circumstances remains unclear. To examine the immunobiologic effects of iv. fDNA injection on the TLR9-mediated autophagy response in murine DSS-colitis. Different types of modified fDNAs were
Athina-Myrto Chioni et al.
Methods in molecular biology (Clifton, N.J.), 1487, 255-267 (2016-12-08)
Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic
Xiu-Li Kuang et al.
BMC neuroscience, 11, 137-137 (2010-10-26)
Neuron-derived neurotrophic factor (NDNF) is evolutionarily well conserved, being present in invertebrate animals such as the nematode, Caenorhabditis elegans, as well as in the fruit fly, Drosophila melanogaster. Multiple cysteines are conserved between species and secondary structure prediction shows that
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