C4482
CelLytic™ Y Cell Lysis Reagent
For yeast cells
Synonym(s):
Yeast cell lysis reagent
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About This Item
Quality Level
form
solution
General description
CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.
Application
CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).
Features and Benefits
- Fast: Cell lysis in just 20 minutes
- Efficient: Three times more efficient than glass beads
- Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
- Gentle: Non-denaturing - preserves biological activity
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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