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94046

Sigma-Aldrich

Sodium phosphate dibasic solution

BioUltra, 0.5 M in H2O

Synonym(s):

Disodium hydrogen phosphate solution, di-Sodium hydrogen phosphate solution

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
7558-79-4
Molecular Weight:
141.96
MDL number:
MFCD00003496
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
57653505
NACRES:
NA.25
Pricing and availability is not currently available.

product line

BioUltra

form

liquid

concentration

0.5 M in H2O

impurities

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.064 g/mL at 20 °C

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic solution has been used:
  • to supplement Dulbecco′s Modified Eagle Medium (DMEM) in calcifying medium to induce the calcification of vascular smooth muscle cells (VSMCs)
  • to prepare a stock solution of CaCl2 and MgCl2 to synthesize calcium phosphate (CPB) and magnesium phosphate bions (MPBs) respectively
  • to supplement 2xYT agar plates to culture BL21 DE3 star cells and BL21dLac Gold cells

Sodium phosphate dibasic solution may be used in the preparation of a wide variety of phosphate based biological buffers for use in cell and molecular biology application.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM4374
BCCL9937
BCCM2829
BCCK4896
BCCJ7861

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Daria Shishkova et al.
International journal of molecular sciences, 21(22) (2020-11-26)
Calciprotein particles (CPPs), which increasingly arise in the circulation during the disorders of mineral homeostasis, represent a double-edged sword protecting the human organism from extraskeletal calcification but potentially causing endothelial dysfunction. Existing models, however, failed to demonstrate the detrimental action
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Journal of proteome research, 14(1), 120-132 (2014-10-21)
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Molecular human reproduction, 21(5), 424-434 (2015-03-04)
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Filis Kazazi-Hyseni et al.
Pharmaceutical research, 31(10), 2844-2856 (2014-05-16)
The aim of this study was the development of poly(D,L-lactide-co-glycolide) (PLGA) microspheres with controlled porosity, to obtain microspheres that afford continuous release of a macromolecular model compound (blue dextran). PLGA microspheres with a size of around 40 μm and narrow size
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Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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