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556416

Sigma-Aldrich

New Methylene Blue N

Dye content ~70 %

Synonym(s):

Basic Blue 24, Methylene Blue N

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S
CAS Number:
1934-16-3
Molecular Weight:
347.91
Colour Index Number:
52030
EC Number:
217-696-9
MDL number:
MFCD00135878
UNSPSC Code:
12171500
PubChem Substance ID:
24879556
NACRES:
NA.47

form

powder, crystals or chunks

composition

Dye content, ~70%

mp

190 °C (dec.) (lit.)

λmax

590 nm
632 nm (2nd)

εmax

≥29000 at 626-634 nm in water
≥30500 at 586-594 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C

InChI

1S/C18H22N3S.ClH/c1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;/h7-10,19-20H,5-6H2,1-4H3;1H/q+1;/p-1

InChI key

NZYCYASKVWSANA-UHFFFAOYSA-M

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Application

New methylene blue N has been used as a photosensitizer for the photodynamic inactivation of Candida albicans.

Biochem/physiol Actions

New methylene blue N can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N helps in counting of reticulocytes (erythrocytes containing RNA).

Features and Benefits

Solvent soluble cationic thiazine dye

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000376807
0000376807
0000164445
SHBN9708
SHBL0614

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Fernando A Castro et al.
Veterinary surgery : VS, 34(6), 539-542 (2005-12-14)
To evaluate the accuracy of a new technique for perineural injection of the lateral palmar nerve and to determine frequency of inadvertent injection into the carpal synovial sheath with this technique. Prospective experimental study. Thirty equine cadaver forelimbs. Each of
M Wainwright et al.
Journal of chemotherapy (Florence, Italy), 13(5), 503-509 (2002-01-05)
The antibacterial activities of Methylene Blue and several of its congeners were measured against Yersinia enterocolitica, a gram-negative pathogen known to exhibit significant growth at 4 degrees C and thus constituting a threat to red blood cell concentrates which are
Immature reticulocyte fraction as a criterion for marrow engraftment. Evaluation of a semi-automated reticulocyte counting method.
H Z Grotto et al.
Clinical and laboratory haematology, 21(4), 285-287 (1999-12-03)
M J Lopez et al.
American journal of veterinary research, 58(7), 786-790 (1997-07-01)
To determine the relation between epidural injectate volume (ml/kg of body weight) and its craniad migration in calves and pigs. 23 neonatal calves and 26 feeder pigs. Animals were randomly assigned to receive different volumes of new methylene blue (NMB
Won-gyun Son et al.
Veterinary anaesthesia and analgesia, 38(5), 510-515 (2011-08-13)
To examine the spread of solution in the epidural space of sternally recumbent dogs. Prospective experimental trial. Animals  Ten healthy adult Beagle dogs weighing 7.6 ± 1.1 kg. Dogs were anaesthetized with total intravenous propofol infusion, and placed in sternal recumbency. A volume
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