MAB5352
Anti-Notch 1 Antibody, clone mN1A
clone mN1A, Chemicon®, from mouse
Synonym(s):
Anti-AOS5, Anti-AOVD1, Anti-TAN1, Anti-hN1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
mN1A, monoclonal
species reactivity
rat, mouse, feline
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... NOTCH1(4851)
General description
Notch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).
Specificity
Recognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.
Immunogen
Cdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.
Application
Anti-Notch 1 Antibody, clone mN1A is a high quality Mouse Monoclonal Antibody for the detection of Notch 1 & has been validated in ICC, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western blot using ECL
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Target description
272.5 kDa (also 230 kDa processed form)
Linkage
Replaces: 04-1046
Physical form
Format: Purified
Protein A purified
Purified immunoglobulin. Liquid.
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
In fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.
In fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shu Li et al.
Molecular medicine reports, 9(6), 2233-2238 (2014-03-20)
Brain arteriovenous malformation (bAVM) is currently one of the most common cerebral vascular diseases, which result in severe clinical outcomes. The Notch signaling pathway is involved in vasculogenesis and angiogenesis, as well as vascular remodeling and arteriovenous differentiation in multiple
Xue-Liang Zhou et al.
Journal of cellular physiology, 234(6), 8834-8845 (2018-10-15)
During myocardial infarction (MI), cardiac fibroblasts (CFs) transform into myofibroblast (CMT). This study aimed to investigate the crosstalk of Notch1 and transforming growth factor-β1 (TGF-β1)/Smad3 signaling in the regulation of CMT and myocardial fibrosis. Primary CFs were isolated from young
Fei Guo et al.
Cell biology international, 36(11), 997-1004 (2012-08-08)
Angiogenesis takes place after brain ischaemia, and stroke-induced angiogenesis in ischaemic brain may be associated with improved neurological recovery. Bone MSCs (marrow stromal cells) transplantation can promote this vital angiogenesis in ischaemic zones, but the mechanisms by which MSCs promoting
Susanne Pettersson et al.
The Biochemical journal, 450(3), 523-536 (2012-12-21)
The Notch receptor is necessary for modulating cell fate decisions throughout development, and aberrant activation of Notch signalling has been associated with many diseases, including tumorigenesis. The E3 ligase MDM2 (murine double minute 2) plays a role in regulating the
Yan-Min Feng et al.
Cell cycle (Georgetown, Tex.), 13(5), 782-791 (2014-01-09)
A critical process of early oogenesis is the entry of mitotic oogonia into meiosis, a cell cycle switch regulated by a complex gene regulatory network. Although Notch pathway is involved in numerous important aspects of oogenesis in invertebrate species, whether
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