GF144

Epidermal Growth Factor Protein, Human recombinant

>98% (SDS-PAGE), recombinant, expressed in E. coli

• Synonym(s): EGF
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Assay: >98% (SDS-PAGE)
• Impurities: <0.1 ng/μg Endotoxin (of EGF; 1EU/μg)

Properties

• Product Name: Epidermal Growth Factor Protein, Human recombinant, The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.
• Quality Level: 100
• assay: >98% (SDS-PAGE)
• specific activity: ≥1 x 10^7 U/mg
• manufacturer/tradename: Chemicon^®
• impurities: <0.1 ng/μg Endotoxin (of EGF; 1EU/μg)
• input: sample type neural stem cell(s); sample type epithelial cells; sample type mesenchymal stem cell(s)
• NCBI accession no.: NM_001963.2
• UniProt accession no.: P01133
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.

Product Source: E.coli

Application

For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.

Physical form

Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.

Preparation Note

The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.

Analysis Note

Specific Activity: Human EGF is fully biologically active when compared to standards.The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL

Other Notes

Replaces: GF001

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3