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Key Documents

AB3080P

Sigma-Aldrich

Anti-Green Fluorescent Protein Antibody

Chemicon®, from rabbit

Synonym(s):

GFP, eGFP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

vertebrates (most common)

species reactivity (predicted by homology)

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Specificity

AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
Reactivity with other species has not been confirmed.

Immunogen

Highly purified native GFP from Aequorea victoria.

Application

Anti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot:
A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.

ELISA:
A 1:800-1:1,000 dilution of a previous lot was used in ELISA.

Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore′s highly purified secondary antibodies in indirect detection systems.

Immunocytochemistry:

Optimal working dilutions and protocols must be determined by end user.

Quality

Routinely evaluated by immunocytochemistry on RENCX cells.

Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot′s anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.

Target description

Varies (GFP is 27 kDa)

Physical form

Affinity purified rabbit immunoglobulin
Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.

Analysis Note

Control
Cells transfected with GFP-tagged fusion vector

RENCX cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Polarized deposition of basement membrane proteins depends on Phosphatidylinositol synthase and the levels of Phosphatidylinositol 4,5-bisphosphate.
Devergne, O; Tsung, K; Barcelo, G; Schupbach, T
Proceedings of the National Academy of Sciences of the USA null
Olivier Devergne et al.
Cell reports, 18(8), 1831-1839 (2017-02-24)
The basement membrane (BM), a sheet of extracellular matrix lining the basal side of epithelia, is essential for epithelial cell function and integrity, yet the mechanisms that control the basal restriction of BM proteins are poorly understood. In epithelial cells
Wanying Zhang et al.
eLife, 8 (2019-02-12)
Layer (L) 2/3 pyramidal neurons in the primary somatosensory cortex (S1) are sparsely active, spontaneously and during sensory stimulation. Long-range inputs from higher areas may gate L2/3 activity. We investigated their in vivo impact by expressing channelrhodopsin in three main
Jason Seth Rothman et al.
eLife, 5 (2016-08-20)
Encoding continuous sensory variables requires sustained synaptic signalling. At several sensory synapses, rapid vesicle supply is achieved via highly mobile vesicles and specialized ribbon structures, but how this is achieved at central synapses without ribbons is unclear. Here we examine
Haewon Byun et al.
The Journal of comparative neurology, 524(11), 2300-2321 (2015-12-30)
The superior colliculus (SC) is a midbrain center involved in controlling head and eye movements in response to inputs from multiple sensory modalities. Visual inputs arise from both the retina and visual cortex and converge onto the superficial layer of

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