G005921

GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB^®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel^™ QuE Z-Sep, Fast GC Analysis

suitable for GC, application for SPE

Properties

• technique(s): gas chromatography (GC): suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: 10 g whole cow′s milk; extraction tube: Supel^™ QuE Non-buffered tube (4 g magnesium sulfate, 1 g sodium chloride) (55294-U); clean-up tube: Supel^™ QuE Z-Sep 150 mg, magnesium sulfate (150 mg, part no. 63135) (55299-U); column: SLB-5ms, 20 m x 0.18 mm I.D., 0.18 μm (28564-U); oven: 75 °C (1 min), 12 °C/min to 340 °C (10 min); inj. temp.: 250 °C; detector: μ-ECD, 340 °C; carrier gas: hydrogen, 1.2 mL/min, constant; injection: 1 μL splitless (0.75 min); liner: 4 mm I.D. FocusLiner^™ with taper
• suitability: application for SPE
• application(s): food and beverages

Description

General description

Reference: Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034

Analysis Note

This application demonstrates the use of dispersive SPE or "QuEChERS" for the extraction and cleanup of whole cow′s milk for GC-ECD analysis of polychlorinated biphenyl (PCBs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >70% for all PCB congeners evaluated except no. 209, which was recovered at 68%.
Extraction solvent: acetone:hexane (1:1), 20 mL
Clean-up volume: 1 mL

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC