Skip to Content
Merck
All Photos(1)

Key Documents

11019AST

Supelco

Astec® CHIROBIOTIC® V Chiral (5 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L88

description

HPLC Column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 2.1 mm

matrix

High-purity silica gel particle platform
fully porous particle

matrix active group

vancomycin phase

particle size

5 μm

pore size

100 Å

operating pH range

3.5-7.0

separation technique

chiral

Looking for similar products? Visit Product Comparison Guide

General description

Astec CHIROBIOTIC® CSPs (chiral stationary phases) interact with ionisable, polar and neutral analytes through multiple molecular interactions. This property makes it widely versatile, thereby making it applicable in a variety of mobile phases. Another feature is the presence of ionic interactions, allowing them to be used reversed-phase and polar ionic modes for sensitive LC-MS operation. CHIROBIOTIC® V is based on bonding Vancomycin which contains 18 chiral centers surrounding three cavities or pockets. These cavities are surrounded with five aromatic rings. Hydrogen acceptor and donor sites are present very near to the ring structures. The CHIROBIOTIC® V is similar to glycoprotein phase′s selectivity wise, with its exception of exhibiting high sample capacity and being stable from 0-100% organic modifier.
Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

  • Bonded phase: Vancomycin
  • Operating pH range: 3.5 - 7.0
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)

Application

Chirobiotic V HPLC column may be used for separating the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

M Kosel et al.
Journal of chromatography. B, Biomedical sciences and applications, 719(1-2), 234-238 (1998-12-30)
A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma. Citalopram, demethylcitalopram and didemethylcitalopram, as well as the internal
Sherri L MacLeod et al.
Journal of chromatography. A, 1170(1-2), 23-33 (2007-10-05)
A reversed-phase enantioselective liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was developed to measure enantiomer fractions (EF) and concentrations of pharmaceuticals in wastewater. Enantiomer resolution of six beta-blockers (atenolol, metoprolol, nadolol, pindolol, propranolol, and sotalol) along with two selective serotonin re-uptake
J P Bagnall et al.
Journal of chromatography. A, 1249, 115-129 (2012-07-04)
This paper presents and compares for the first time two chiral LC-QTOF-MS methodologies (utilising CBH and Chirobiotic V columns with cellobiohydrolase and vancomycin as chiral selectors) for the quantification of amphetamine, methamphetamine, MDA (methylenedioxyamphetamine), MDMA (methylenedioxymethamphetamine), propranolol, atenolol, metoprolol, fluoxetine
Ana R Ribeiro et al.
Chemosphere, 95, 589-596 (2013-11-05)
Microbial degradation is the most important process to remove organic pollutants in Waste Water Treatment Plants. Regarding chiral compounds this process is normally enantioselective and needs the suitable analytical methodology to follow the removal of both enantiomers in an accurate
Chiral stability-indicating HPLC method for analysis of arotinolol in pharmaceutical formulation and human plasma
Sultan, Maha A., et al.
Arabian Journal of Chemistry, 3 (3), 147-153 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service