SRP6014

HGF human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonym(s): F-TCF, HGF, HGFB, Hepatopoietin (HPTA), Scatter Factor (SF)
• CAS Number: 953836-17-4
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in CHO cells
• Assay: ≥97% (HPLC); ≥97% (SDS-PAGE)
• form: lyophilized
• mol wt: 80.017 kDa
• packaging: pkg of 1 mg; pkg of 10 μg
• technique(s): cell culture | embryo: suitable
• impurities: Endotoxin, tested
• NCBI accession no.: P14210
• UniProt accession no.: P14210
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... HGF(3082)

Description

General description

Hepatocyte growth factor (HGF) is a multifunctional growth factor. HGF Human Recombinant produced in Chinese hamster ovary (CHO) is a heterodimer, non-glycosylated, polypeptide chain consisting of an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017kDa. The gene encoding this protein is localized on human chromosome 7q21.1. The HGF is purified by proprietary chromatographic techniques.

Biochem/physiol Actions

Hepatocyte growth factor (HGF) regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with interleukin-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. It is expressed in brain tumors.

Physical form

The protein was lyophilized from a concentrated (1 mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.

Reconstitution

Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18 MΩcm^-1 H₂O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable