Skip to Content
Merck
All Photos(2)

Documents

SRP5013

Sigma-Aldrich

CK1 α 1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

CK1, CK1A1, CSNK1A1, HLCDGP1, PRO2975

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

102-138 nmol/min·mg

mol wt

~62 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CSNK1A1(1452)

General description

Casein kinase 1 α1 (CK1α1) is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes. CK1α1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1α1 phosphorylates Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal. CK1α1 dynamically associates with the CARMA1-BCL10-MALT1 (CBM) complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1α1 can form complex with the E3 ubiquitin-protein ligase MDM2, which then regulates the stability of p53 and E2F-1 transcription factors.

Application

GST tagged active CK1α1 has been used in downstream coprecipitation and mass spectrometry to demonstrate the phosphorylation of the Wnt regulator, Jade-1, by CK1α1.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Fused?Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation
Genes & Development (2007)
Oncogenic RAS-induced CK1a drives nuclear FOXO
proteolysis
F. Zhang
Oncogene, 37 (2018)
Casein Kinase 1 a Phosphorylates the Wnt Regulator Jade-1 and Modulates Its Activity*
Lori Borgal
The Journal of Biological Chemistry (2014)
The role of the casein kinase 1 (CK1) family in different signaling pathways linked to cancer development.
Knippschild U
Onkologie (2005)
AIP augments CARMA1-BCL10-MALT1 complex formation to facilitate NF-?B signaling upon T cell activation
Cell communication and signaling : CCS (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service