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P8169

Sigma-Aldrich

Phytagel™

suitable for plant cell culture, BioReagent, powder

Synonym(s):

Gellan Gum, Agar substitute gelling agent

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About This Item

CAS Number:
71010-52-1
EC Number:
275-117-5
MDL number:
MFCD00131909
UNSPSC Code:
10171502
NACRES:
NA.72

product line

BioReagent

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

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General description

Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.

Application

Phytagel™ has been used:
  • in the solidification of culture medium for root assays in Arabidopsis thaliana
  • as a component of Solid Paul′s medium for Agrobacterium culture
  • as a component of root induction medium in Ilex dumosa tissue culture

Biochem/physiol Actions

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Reconstitution

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Luna C, et al.
Biocell, 27(2), 205-212 (2003)
CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants
Delay C, et al.
Journal of Experimental Botany, 64(17), 5383-5394 (2013)
Abhishek Bhaskaran et al.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 19(5), 874-880 (2016-05-22)
Longer procedural time is associated with complications in radiofrequency atrial fibrillation ablation. We sought to reduce ablation time and thereby potentially reduce complications. The aim was to compare the dimensions and complications of 40 W/30 s setting to that of
Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells
Buschmann H, et al.
The New phytologist, 190(1), 258-267 (2011)
Yasuka L Yamaguchi et al.
Frontiers in plant science, 8, 1195-1195 (2017-07-28)
Developmental plasticity is one of the most striking features of plant morphogenesis, as plants are able to vary their shapes in response to environmental cues. Biotic or abiotic stimuli often promote organogenesis events in plants not observed under normal growth
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Protocols

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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