H7006

HEPES sodium salt

≥99.0% (titration)

• Synonym(s): Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• EC Number: 278-169-7
• MDL number: MFCD00036463
• UNSPSC Code: 12161700
• PubChem Substance ID: 24895780
• NACRES: NA.25

Properties

• Quality Level: 400
• Assay: ≥99.0% (titration)
• form: powder
• storage condition: dry at room temperature
• technique(s): HPLC: suitable
• color: white
• pH: 10-12 (1 M in water)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 0.1 M, clear, colorless
• suitability: suitable for mass spectrometry
• application(s): diagnostic assay manufacturing; general analytical (; ); life science and biopharma; metabolomics
• storage temp.: room temp
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

General description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Application

HEPES buffer finds application in:

• Most studies with metal ions
• Substitute for Tris and phosphate in studies with metal ions
• Grinding buffer in plant studies
• Running buffer in gel electrophoresis
• Buffer in electroporation studies
• Bradford or bicinchoninic acid (BCA) assays
• Extraction buffer to prevent damage of proteins in red blood cells

It has been used:

• as a buffer component in the preparation of a low sucrose buffer[1]
• as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions[2]

Features and Benefits

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Highly soluble in water
• Negligible metal ion binding
• Less toxic to cells than other buffers such as Tris and phosphate
• Stable in a wide pH range

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

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Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves