D2158

Histodenz^™

nonionic density gradient medium

• Synonym(s): 5-(N-2,3-Dihydroxypropylacetamido)-2,4,6-triiodo-N,N′-bis(2,3-dihydroxypropyl)isophthalamide
• Empirical Formula (Hill Notation): C₁₉H₂₆I₃N₃O₉
• CAS Number: 66108-95-0
• Molecular Weight: 821.14
• Beilstein: 2406632
• EC Number: 266-164-2
• MDL number: MFCD00077732
• UNSPSC Code: 12352207
• PubChem Substance ID: 24893646
• NACRES: NA.75

Properties

• Quality Level: 200
• form: powder
• application(s): hematology; histology
• storage temp.: room temp
• SMILES string: CC(=O)N(CC(O)CO)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I
• InChI: 1S/C19H26I3N3O9/c1-8(29)25(4-11(32)7-28)17-15(21)12(18(33)23-2-9(30)5-26)14(20)13(16(17)22)19(34)24-3-10(31)6-27/h9-11,26-28,30-32H,2-7H2,1H3,(H,23,33)(H,24,34)
• InChI key: NTHXOOBQLCIOLC-UHFFFAOYSA-N

Description

General description

HistoDenz^™, also known as iohexol, is a non-ionic X-ray contrast compound developed specifically as an iodinated density gradient medium.[1]

Application

HistoDenz^™ is suitable for separating everything from macromolecules to viable cells by either rate-zonal or isopycnic centrifugation.

• It has been used in the isolation and culturing of hepatic stellate cells.[2]
• It has also been employed in the extraction of microbial cells from environmental samples.[3]
• It has been used in LacZ (β-galactosidase) staining.[4]
• It has also been used for the enrichment of gonocytes.[5]

Features and Benefits

HistoDenz^™ offers several favorable properties as a gradient medium.

• It has very low toxicity, low osmolality, high density, and low viscosity (compared to sucrose).
• It is water-soluble, non-ionic, and chemically near-inert.[6]

Legal Information

Histodenz is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves