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CRISPR18V

Sigma-Aldrich

Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

form

liquid

Quality Level

packaging

vial of 200 μL

concentration

(1x106 TU/ml (via p24 titering assay))

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

General description

The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Application

Functional Genomics/Screening/Target Validation

Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

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