Product B1911 is not formulated as a balanced salt solution. Therefore, it should be applied to fixed cells, as live cells are not suitable for staining with this solution.
B1911
BCIP®/NBT
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Pricing and availability is not currently available.
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Product Name
BCIP®/NBT Liquid Substrate System, ready to use solution
Quality Level
form
liquid
storage temp.
2-8°C
Application
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
- for staining alkaline phosphatase(ALP) in bone marrow stromal cells[1]
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody[2]
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays[3]
Caution
It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocols
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
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Should this be applied to fixed cells or live cells for staining?
1 answer-
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Is the BCIP in your BCIP/NBT Liquid Substrate part number B1911 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt?
1 answer-
The BCIP used in Product B1911 is not 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt (CAS# 6578-06-9).
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