A5106

Anti-ABI1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Abelson interactor 1, Anti-Abl-interactor protein 1, Anti-Nap1 binding protein NAP1BP, Anti-eps8 binding protein, Anti-interactor protein AblBP4, Anti-spectrin SH3 domain binding protein 1 SSH3BP1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~65 kDa
• species reactivity: human, canine (predicted), mouse (predicted), rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 0.5-1.0 μg/mL using A431 cell lysate; western blot: 0.5-1.0 μg/mL using extract of rat brain cytosolic fraction (S1)
• UniProt accession no.: Q8IZP0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ABI1(10006) ; mouse ... Abi1(11308) ; rat ... Abi1(79249)

Description

General description

Abelson interactor 1 (Abi1) protein, a 65 kD-substrate of the eponymous Abelson non-receptor tyrosine kinase (c-Abl) is expressed mainly in the central nervous system. This gene is located on human chromosome 10p12.

Abi 1 is an adaptor protein that interacts with Wave2 to regulate actin polymerization.

Immunogen

synthetic peptide corresponding to amino acids 163-180 of human ABI1, conjugated to KLH. The corresponding sequence is identical in several species including mouse, rat, and dog.

Application

Anti-ABI1 antibody produced in rabbit has been used in western blot analysis and immunofluorescence.

Rabbit anti- ABI1 antibody can be used for immunoblotting assays at a concentration of 0.5-1.0μg/ml using extracts from A431 and rat brain cells.

Biochem/physiol Actions

Abelson interactor 1 (Abi1) is essential for the formation and activation of WAVE2 signaling complex. ABI1 and WAVE are essential for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP regulate actin-based vesicular transport, EGF receptor endocytosis, and EGFR and transferrin receptor (TfR) cell surface distribution. ABI1 has been shown to act as dual regulator of WAVE and neural Wiskott-Aldrich syndrome protein (N-WASP) specific activities. ABI1 is an essential component of this signaling complex. It positively regulates WAVE activity and connects WAVE to Rac through assembly of the WAVE-ABI1-Nap1-PIR121 complex. Abi1 modulates actin reorganization and lamellipodia formation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves