Item B3883 can be stored at room temperature.
B3883
Bile bovine
dried, unfractionated
Synonym(s):
Ox gall powder
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About This Item
Pricing and availability is not currently available.
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sterility
non-sterile
Quality Level
form
dried, unfractionated
color
yellow-brown
solubility
water: soluble 50 mg/mL
application(s)
microbiology
General description
Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.
Application
Bile bovine has been used:
- in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition[1]
- as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology[2]
- as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583[3]
Biochem/physiol Actions
Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.[4]
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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WHAT IS THE PROPER TEMPERATURE FOR STORAGE?
1 answer-
Helpful?
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Can you tell us the exact bile salt concentration?
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.
Helpful?
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I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.
Helpful?
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