11388908910

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

• Synonym(s): Biotin-16-UTP, biotin
• UNSPSC Code: 41116100

Properties

• Assay: 97.7% (HPLC)
• Quality Level: 100
• form: solution
• mol wt: M_r 987.5 (biotin-16-UTP-Li₄)
• packaging: pkg of 25 μL (250 nmol; 10mM)
• manufacturer/tradename: Roche
• color: colorless
• solubility: water: miscible
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Application

Biotin-labeled RNA can be used as a hybridization probe for:

• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization

The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Quality

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

Physical form

Biotin-16-UTP, tetralithium salt, 10mM solution

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash