MABF3090
Anti-SARS-CoV-2 Spike N501Y Antibody, clone 6D4-E2
Synonym(s):
S glycoprotein Alpha N501Y, Spike glycoprotein Alpha N501Y
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
6D4-E2, monoclonal
mol wt
calculated mol wt 141.18 kDa
observed mol wt ~200 kDa
purified by
using protein G
species reactivity
virus
packaging
antibody small pack of 100 μL
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
1 of 4
This Item | MABF2784 | MABF2783 | ABF1064 |
---|---|---|---|
6D4-E2, monoclonal | 6E4-C5, monoclonal | 8B12-C2, monoclonal | polyclonal |
mouse | mouse | mouse | rabbit |
purified antibody | purified antibody | purified antibody | purified antibody, serum |
immunoprecipitation (IP): suitable, western blot: suitable | ELISA: suitable, immunoprecipitation (IP): suitable, neutralization: suitable, western blot: suitable | ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable | ELISA: suitable, neutralization: suitable, western blot: suitable |
2-8°C | - | - | - |
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from HEK293T cells transiently transfected with SARS-CoV-2 spike B.1.351, beta.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SARS-CoV-2 spike protein with N501Y mutation in lysate from HEK293T cells transiently transfected with SARS-CoV-2 spike B.1.351, Beta, but not in wild-type HEK293T cells.
Tested Applications
Western Blotting Analysis: A representative lot detected SARS-CoV-2 spike N501Y in HEK293T transiently transfected with various SARS-CoV-2 variants (Data courtesy of Stefan Schüchner and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot detected SARS-CoV-2 spike N501Y in Whole cell lysates of HEK293T cells (untransfected or transiently transfected with SARS-CoV-2 spike WT or B.1.351 variant) (Data courtesy of Stefan Schüchner and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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