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MAB1864

Sigma-Aldrich

Anti-TUBA1A (TUBA3) Antibody

CHEMICON®, rat monoclonal, YL1/2

Synonym(s):

Anti-B-ALPHA-1, Anti-LIS3, Anti-TUBA3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

Product Name

Anti-Tubulin Antibody, clone YL1/2, clone YL1/2, Chemicon®, from rat

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

YL1/2, monoclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

Q71U36

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

General description

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Specificity

MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.

Immunogen

Epitope: The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Yeast tubulin

Application

Detect Tubulin using this Anti-Tubulin Antibody, clone YL1/2 validated for use in ELISA, IH, IP, RIA & WB.
Immunohistochemistry:
1:50-1:100 dilutoin from a previous lot was used on frozen sections.

ELISA:
1:100-1:1,000 dilution from a previousl lot was used.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Routinely evaluated by Western Blot on A431 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.

Target description

55 kDa

Linkage

Replaces: 04-1117

Physical form

Format: Purified
Protein A purified
Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide.

Storage and Stability

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
All tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.
Williams, LS; Ganguly, S; Loiseau, P; Ng, BF; Palacios, IM
Development null
Integrin alpha 6 beta 4 promotes migration, invasion through Tiam1 upregulation, and subsequent Rac activation.
Cruz-Monserrate, Z; O'Connor, KL
Neoplasia null
Peter T Ruane et al.
Scientific reports, 6, 27456-27456 (2016-06-09)
Microtubules and their associated proteins (MAPs) underpin the polarity of specialised cells. Adenomatous polyposis coli (APC) is one such MAP with a multifunctional agenda that requires precise intracellular localisations. Although APC has been found to associate with kinesin-2 subfamily members
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
Lee, S; Liu, HP; Lin, WY; Guo, H; Lu, B
The Journal of Neuroscience null
Interphase cytofission maintains genomic integrity of human cells after failed cytokinesis.
Choudhary, A; Lera, RF; Martowicz, ML; Oxendine, K; Laffin, JJ; Weaver, BA; Burkard, ME
Proceedings of the National Academy of Sciences of the USA null
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Questions

  1. Hello, how should this antibody be stored for long term? Should glycerol be added for long-term storage at -20C?

    1 answer

    1. As mentioned in the 'DESCRIPTION' section under 'Storage and Stability' this product is stable for at least 6 months from date of receipt when stored at -20°C in undiluted aliquots.

      Upon the first thaw, and before removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

      Helpful?

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