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1.00485

Sigma-Aldrich

Masson-Goldner staining kit

for the visualization of connective tissue with trichromic staining

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About This Item

UNSPSC Code:
41116124
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

400

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

Related Categories

General description

The Masson-Goldner staining kit - for the visualization of connective tissue with trichromic staining, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. Using a combination of three different staining solutions, muscle fibers, collagenous fibers, fibrin and erythrocytes can be selectively visualized.
The original methods were primarily used to differentiate collagenous and muscle fibers. The stains used have different molecular sizes and enable the individual tissues to be stained differentially.
The Masson-Goldner staining technique can be carried out using formalin fixed material. Subsequent to staining the nucleus with Weigert′s iron hematoxylin, components such as muscle, cytoplasm and erythrocytes are stained with azophloxin and orange G solution. Connective tissue is then counter stained using light green SF solution.
The package is sufficient for 400 - 500 applications. The product is registered as IVD and CE product and can be used in diagnostics and laboratory accreditation. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy (Tissue section): passes test
Nuclei: dark brown to black
Cytoplasm: brick red
musculature (muscles): bright red
Connective tissue: green
acid mucosubstances: green
Erythrocytes: bright orange

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H315 - H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aurora Garre et al.
Clinical, cosmetic and investigational dermatology, 11, 253-263 (2018-06-09)
With age, decreasing dermal levels of proteoglycans, collagen, and elastin lead to the appearance of aged skin. Oxidation, largely driven by environmental factors, plays a central role. The aim of this study was to assess the antiaging efficacy of a
Álvaro Santana-Garrido et al.
Journal of physiology and biochemistry (2022-08-10)
Arterial hypertension (AH) leads to oxidative and inflammatory imbalance that contribute to fibrosis development in many target organs. Here, we aimed to highlight the harmful effects of severe AH in the cornea. Our experimental model was established by administration of
B Shen et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 41(7), 1107-1113 (2021-07-27)
To investigate the effect of ginsenoside Rh2 (G-Rh2) on renal fibrosis and cell apoptosis in rats with diabetic nephropathy (DN) and explore its possible mechanism. Thirty male SD rats were randomized equally into control group, DN group and ginsenoside Rh2
Wenjiao Li et al.
Polymers, 14(13) (2022-07-10)
Various congenital and acquired urinary system abnormalities can cause structural damage to patients' bladders. This study aimed to construct and evaluate a novel surgical patch encapsulated with adipose-derived stem cells (ADSCs) for bladder tissue regeneration. The surgical patch consists of
Huan He et al.
Redox biology, 48, 102185-102185 (2021-11-15)
Reports indicate that the mechanism of doxorubicin (Dox)-induced cardiotoxicity is very complex, involving multiple regulatory cell death forms. Furthermore, the clinical intervention effect is not ideal. Iron dependence, abnormal lipid metabolism, and excess reactive oxygen species generation, three characteristics of

Questions

1–3 of 3 Questions  

  1. Can I use this kit to visualize fibrosis?

    1 answer

    1. The Goldner Trichrome kit can be one of the tests performed to aid in the diagnosis of various types of fibrosis. Blood testing is sometimes used to rule out other diseases. Imaging tests may be used if pulmonary fibrosis is expected. A hematoxylin and eosin stain may be performed initially.

      Fibrosis is a disease where interstitial tissue overgrows, hardens, and scars. This leads to chronic inflammation. The various Trichrome methods (Goldner, Goldner Masson, Gomori and Masson) can be used. The various trichrome methods typically identify muscle and collagen. Interstitial tissue is composed of collagen, elastin, and glycosaminoglycans. The Goldner Masson Trichrome procedure also identifies fibrin and red blood cells.

      Helpful?

  2. Hello, I wonder what is the difference of theTrichrome Stain (Masson) Kit (HT15) and Masson-Goldner staining kit (1.00485.0001)? Thanks

    1 answer

    1. The 1.00485.0001, Masson-Goldner Kit, is primarily used for the visualization of connective tissue with trichromic staining. This kit Includes a phosphotungstate reagent, a light green dye to stain collagen/connective tissues, and Azophloxine(as described by Goldner).
      The HT15, Trichrome (Masson) Kit, is intended for use in the study of connective tissue, muscle, and collagen fibers. This kit includes both, a phosphotungstate and a phosphomolybdate component, Aniline blue to specifically stain collagen, and Biebrich Scarlet – Acid Fuchsin.
      Both kits require the use of Weigert's Iron Hematoxylin, which is not included.

      For a more comprehensive understanding of the kits, please see the links below to review the product datasheets:
      HT15 Trichrome (Masson) kit
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/274/001/procedure-ht15ug-ms.pdf

      1.00485.0001 Masson-Goldner kit
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/226/100485-en.pdf

      Helpful?

  3. Hi, Can I use this kit for osteoid investigation?

    1 answer

    1. Although it is not specifically tested for suitability in osteoid studies, researchers have used it successfully in this application. Please review this peer-reviewed article: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/806/engineering-bone-tissue-from-human-embryonic-stem-cells.pdf

      Helpful?

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