202096

New Methylene Blue N zinc chloride double salt

Dye content 90 %

• Synonym(s): Basic blue 24, Methylene blue N
• Empirical Formula (Hill Notation): C₁₈H₂₂ClN₃S · 0.5ZnCl₂
• CAS Number: 6586-05-6
• Molecular Weight: 416.05
• Colour Index Number: 52030
• EC Number: 229-516-6
• MDL number: MFCD00078135
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852036
• NACRES: NA.47

Properties

• form: powder
• composition: Dye content, 90%
• mp: >240 °C (dec.) (lit.)
• λmax: 591 nm; 630 nm (2nd)
• ε (extinction coefficient): ≥16000 at 242-248 nm in H₂O; ≥28000 at 284-290 nm in H₂O; ≥36000 at 588-594 nm in H₂O; ≥44000 at 629-635 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Cl-].[Cl-].[Cl-].Cl[Zn]Cl.CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C.CCNc4cc5[s+]c6cc(NCC)c(C)cc6nc5cc4C
• InChI: 1S/2C18H22N3S.4ClH.Zn/c2*1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;;;;;/h2*7-10,19-20H,5-6H2,1-4H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: MCBJJSJOIVJYQY-UHFFFAOYSA-J

Description

Application

New Methylene Blue N zinc chloride double salt has been used as a photosensitizer for the in vitro photodynamic inactivation of plant-pathogenic fungi Colletotrichum acutatum and Colletotrichum gloeosporioides. It has also been used to determine the in vitro susceptibilities of Trichophyton mentagrophytes and Trichophyton rubrum microconidia to photodynamic antimicrobial chemotherapy (PACT).

Biochem/physiol Actions

New Methylene Blue N zinc chloride double salt also known as methylene blue N is an azo dye. It can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living, young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N zinc chloride double salt helps in counting of reticulocytes (erythrocytes containing RNA).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves