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S9883

Millipore

Sea salts

NutriSelect® Basic

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85
Pricing and availability is not currently available.

sterility

non-sterile

Quality Level

200

form

powder

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

application(s)

agriculture
environmental
food and beverages
life science and biopharma

suitability

marine bacteria

General description

Sea salts are unrefined salt, which is harvested from ocean and saltwater deposit.[1] It is used to maintain the body′s salt balance.[2]

Application

Sea salts has been used:
  • for the preparation of artificial seawater to culture microalgae[3][4]
  • to assess nitrogen fixation rates[5]
  • as a supplement in Hugh and Leifson′s oxidation fermentation (OF) basal medium[6][7]

Usually used at approx. 40 g/L for culturing marine bacteria.

Other Notes

An artificial salt mixture closely resembling the composition of the dissolved salts of ocean water.

Preparation Note

Usually used at ~ 40 g/L for culturing marine bacteria.
After dissolving, adjust pH to 8.2 using 0.1 N solution of sodium hydroxide or hydrochloric acid.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLBX8504
SLBX3050
SLBM4583V
SLBM4545V
MKBR5550V

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Biomass and oil production by Chlorella vulgaris and four other microalgae?Effects of salinity and other factors
Luangpipat T and Chisti Y
Journal of Biotechnology, 257, 47-57 (2017)
Winogradskyella sediminis sp. nov., isolated from marine sediment
Zhang DC, et al.
International Journal of Systematic and Evolutionary Microbiology, 66(8), 3157-3163 (2016)
Matthias Wietz et al.
Marine drugs, 8(12), 2946-2960 (2011-02-23)
On a global research expedition, over 500 bacterial strains inhibitory towards pathogenic bacteria were isolated. Three hundred of the antibacterial strains were assigned to the Vibrionaceae family. The purpose of the present study was to investigate the phylogeny and bioactivity
Identification and analysis of OsttaDSP, a phosphoglucan phosphatase from Ostreococcus tauri
Carrillo JB, et al.
PLoS ONE, 13(1), e0191621-e0191621 (2018)
Marinomonas mangrovi sp. nov., isolated from mangrove sediment
Zhang DC and Margesin R
International Journal of Systematic and Evolutionary Microbiology, 65(5), 1537-1541 (2015)
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Can the sea salts (S9883) be used to prepare f/2 medium?

    1 answer

    1. Yes, this product can be used in F/2 medium for culturing marine microbes. See the attachments and link below.
      https://www.sciencedirect.com/science/article/pii/S221192641830242X
      https://www.doc-developpement-durable.org/file/Culture/culture-algues/algoculture/alga%20culturing%20techniques%20Harrison_Berges_2005.pdf

      Helpful?

  2. I am using this to make synthetic seawater for membrane separations. I am not sure what amount of salt do I put into how much pure water to have an exact concentration of seawater. The specs mention <0.5ppm of trace metals, does this include Lithium?

    1 answer

    1. The recommended usage concentration for marine culture is 40 g/L in H2O. The trace metals in this product will be found on the lot specific Certificate of Analysis. Please view the link below:
      https://www.sigmaaldrich.com/certificates/COFA/S9/S9883/S9883-BULK________SLBX8504__.pdf

      Lithiium is not an element that is regularly screened. Historically, the reported lithium concentration has been approximately 0.18 MG/L.

      Helpful?

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