O4387

Oligo(dT)₂₃, Anchored

70 μM in H₂O

• Synonym(s): Oligo(dT)23 Anchored Primer
• UNSPSC Code: 41106305
• NACRES: NA.52

Properties

• biological source: synthetic (organic)
• form: solution
• usage: 0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)
• concentration: 70 μM in H₂O
• color: colorless
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence. The optimal conditions for the concentration of enhanced AMV reverse transcriptase, template RNA, primers, and amplification parameters will depend on the system being utilized and should be determined empirically.

Application

Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).

The Anchored Oligo(dT)₂₃ Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)₂₃ primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)₂₃ primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Features and Benefits

• In the process of preparing cDNA libraries, when there is incomplete or missing sequence information or when specific primers are not suitable, Oligo(dT)23, Anchored primers can be employed together with random nonamers (Product No. R 7647).
• These primers can serve as a substitute for reverse transcription primers in tasks like first-strand cDNA synthesis, cDNA library construction, and various other applications.
• Following transcription, the anchored oligo(dT)23 primers exhibit a reduced ability to initiate priming at higher temperatures (up to 65 °C), ensuring that they do not interfere with PCR.
• Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Other Notes

For laboratory use only. Not for drug, household or other uses.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves