D0443
Dextranase from Chaetomium erraticum
Synonym(s):
Dextranase Plus L, 1,6-α-D-Glucan 6-glucanohydrolase
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General description
A product of Novozymes Corp.
Dextranase is generated by many microorganisms, including bacteria, filamentous fungi, and yeast.
Application
Dextranase from Chaetomium erraticum has been used:
- in microcarrier dissolution to produce multicellular 3D spheroids for drug discovery
- in in vitro digestion of extracellular polysaccharide (EPS) from the bacterial isolates
- in a study to investigate the immobilization of dextranase
- to investigate the optimization of process conditions for enzymatic modification of alternan
Biochem/physiol Actions
Dextranase is an endodextranase that hydrolyzes α-(1,6)-glycosidic linkages in dextran. It has many industrial and clinical applications such as food processing, sugar modification, and medicine preparation. Dextranase is widely used to produce alternated sugar beet and isomaltooligosaccharides (IMOs), a prebiotic functional food. The low molecular weight dextran obtained from dextranase serves as a synthetic blood volume expander. Dextranase can be coupled to antibodies for the detection of tumor cells. It prevents the occurrence of dental plaques by depolymerizing microbial dextran deposits in teeth. Hence, it is used in dental care products.
Preparation Note
A fungal dextranase produced by submerged fermentation of Chaetomium erraticum.
Analysis Note
Stable in the pH range of 3-7 and at temperatures up to approx. 70 °C. For most applications, the preferred conditions are pH 5-6 and a temperature of 50-60 °C.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Kinetics and thermodynamic of the purified dextranase from Chaetomium erraticum
Virgen-Ortiz JJ, et al.
Journal of Molecular Catalysis. B, Enzymatic, 122, 80-86 (2015)
Frank Alwin Erhardt et al.
Journal of biotechnology, 131(4), 440-447 (2007-09-19)
In order to facilitate the Co-Immobilization of dextransucrase and dextranase, various techniques for the immobilization of industrial endo-dextranase from Chaetomium erraticum (Novozymes A/S) were researched. Adsorption isotherms at various pH-values have been determined for bentonite (Montmorillonite), hydroxyapatite and Streamline DEAE.
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