CCRF-CEM

85112105, human blood, Lymphoblast

• UNSPSC Code: 41106514

Properties

• Product Name: CCRF-CEM, 85112105, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85112105, human blood, Lymphoblast
• biological source: human blood
• packaging: tube of 5 μg 85112105-DNA-5UG; pkg of vial of cells 85112105-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian acute lymphoblastic leukaemia

Cell Line Description

A T lymphoblastoid line obtained from the peripheral blood of a 4 year old Caucasian female with acute lymphoblastoid leukaemia. These may be grown to a high density in a spinner type suspension culture. Cells are reported to be free of virus particles as determined by electron microscopy.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-3x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.