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C5914

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Dissociation Solution

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

6.9-7.1

shipped in

wet ice

storage temp.

2-8°C

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General description

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.

Application

Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture, U87 glioblastoma tumor cells and cardiac stem cells (CSCs).
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target

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