B6916
Bradford Reagent
for 0.1-1.4 mg/ml protein
Synonym(s):
Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent
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About This Item
UNSPSC Code:
12161500
NACRES:
NA.32
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General description
Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.
Application
Bradford Reagent has been used to determine total protein concentration.
Features and Benefits
- The reagent is ready to use. No mixing or dilution required.
- Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
- Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
- Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
- Can be used in microwell plate assays.
- Inexpensive assay.
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Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
Target Organs
Eyes,Central nervous system
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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