B3679
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Recommended Products
product name
BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate
Quality Level
form
liquid
storage temp.
2-8°C
Application
BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Caution
Stable at least one year at 2-8 °C.
Physical form
Ready-to-use.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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