A1076

BAPTA-AM

≥95% (HPLC), powder, intracellular Ca²⁺ store chelator

• Synonym(s): 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)
• Empirical Formula (Hill Notation): C₃₄H₄₀N₂O₁₈
• CAS Number: 126150-97-8
• Molecular Weight: 764.68
• Beilstein: 7458534
• MDL number: MFCD00036696
• UNSPSC Code: 12352200
• PubChem Substance ID: 24890502
• NACRES: NA.77

Properties

• Product Name: BAPTA-AM, ≥95% (HPLC)
• Assay: ≥95% (HPLC)
• form: powder
• storage temp.: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccccc1OCCOc2ccccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O
• InChI: 1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3
• InChI key: YJIYWYAMZFVECX-UHFFFAOYSA-N

Description

General description

BAPTA-AM inhibits free radical-mediated toxicity, enhances apoptosis in non-neuronal cells and protects neurons from ischemic damage. It regulates ion channels and blocks neuronal Ca²⁺-activated K⁺ channel currents. BAPTA-AM maintains intracellular Ca²⁺ homeostasis.

Application

BAPTA-AM has been used :

• to reduce the elevation of cleaved caspase-3
• to block cell death, inhibit ROS (reactive oxygen species) production and inhibit caspase-8 activation
• to exclude the effect of changes in intracellular Ca²⁺ ([Ca²⁺]_i) concentration during seizure induction
• to eliminate the increase of [Ca²⁺]_i induced by 6-hydroxydopamine (6-OHDA)

Biochem/physiol Actions

Selective chelator of intracellular Ca²⁺ stores.

Linkage

Membrane-permeable form of BAPTA.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves