94280

Urease from Canavalia ensiformis (Jack bean)

powder, ~1 U/mg

• Synonym(s): Jack bean urease, Urea amidohydrolase
• CAS Number: 9002-13-5
• Enzyme Commission number: 3.5.1.5 (BRENDA, IUBMB)
• EC Number: 232-656-0
• MDL number: MFCD00070858
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• form: powder
• specific activity: ~1 U/mg
• mol wt: M_r ~480000
• storage temp.: −20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.

Application

Application in (selective) hydrolysis/condensations;citation structurecitationcitation.

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.

Other Notes

Application in (selective) hydrolysis/condensations; structure.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves