N2125

Naphthol AS-BI phosphate

≥98% purity (HPLC), powder

• Synonym(s): 7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate
• Empirical Formula (Hill Notation): C₁₈H₁₅BrNO₆P
• CAS Number: 1919-91-1
• Molecular Weight: 452.19
• Beilstein: 6668526
• EC Number: 217-645-0
• MDL number: MFCD00004058
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897552
• NACRES: NA.47

Properties

• product name: Naphthol AS-BI phosphate, ≥98% (HPLC), Technical grade
• Quality Level: 200
• Assay: ≥98% (HPLC)
• form: powder
• color: white to light yellow
• solubility: methanol: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O
• InChI: 1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
• InChI key: HUXIAXQSTATULQ-UHFFFAOYSA-N

Description

General description

Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.

Application

Naphthol AS-BI phosphate is suitable to use:

• in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
• as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
• to perform alkaline phosphatase reaction

Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Biochem/physiol Actions

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Substrates

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves