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12398

Millipore

CP ChromoSelect Agar

NutriSelect® Basic, suitable for microbiology

Synonym(s):

CPC Agar, Clostridium perfringens ChromoSelect Agar, Clostridium perfringens Chromogen Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
ammonium iron(III) citrate, 0.2 g/L
chromogenic mixture, 1.73 g/L
L-cysteine hydrochloride, 1.0 g/L
magnesiumsulfate heptahydrate, 0.1 g/L
soy peptone, 5.0 g/L
sucrose, 3.0 g/L
tris buffer, 1.8 g/L
tryptose (vegetable), 20.0 g/L
yeast extract, 15.0 g/L

manufacturer/tradename

NutriSelect® Basic

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Clostridium spp.

Application

A chromogenic Agar for enumeration detection and differentiation of Clostridium sp., in particular Clostridium perfringens, in aqueous samples. The medium is more reliable than m-CP Agar and easier to handle.

Preparation Note

Suspend 31.42 g in 400 ml distilled water and adjust the volume to 500 ml. Boil to dissolve the medium completely. Cool to 50-55°C and add 1 vial of Perfringens T.S.C. Supplement (P9352) or 1 vials of M-CP selective Supplement I (Cat. No. 51962). Dispense into petri dishes (we recommend small ones).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH0873
BCCB6792
BCBS7424V
BCBM3929V
1446140V

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D P Sartory et al.
Letters in applied microbiology, 27(6), 323-327 (1999-01-01)
Two media (mCP medium and Tryptose Sulphite Cycloserine (TSC) agar) were evaluated for recovery of Clostridium perfringens in environmental and part-treated drinking water. For laboratory strains of Clostridium, mCP was more selective and specific for Cl. perfringens than TSC, but
Epsilon toxin is essential for the virulence of Clostridium perfringens type D infection in sheep, goats, and mice.
Garcia JP
Infection and Immunity, 81 (7), 2405-2414 (2013)
Mammad Manafi et al.
International journal of food microbiology, 167(1), 92-95 (2013-07-03)
The European Directive on drinking water quality has included mCP agar as the reference method for recovering Clostridium perfringens from drinking waters. In the present study, three media (mCP, TSCF and CP Chromo Select Agar) were evaluated for recovery of

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